Paulo Eduardo Martins Ribolla I Universidade Estadual Paulista (UN ESP)
Despite the RNAi consolidation as viable alternative in insect management worldwide, this biopesticide methodology remains unexplored in Brazilian conditions. Therefore, this project aims to develop new targets for the use of shRNA as biopesticides against tropical diseases vectors as Anopheles, Aedes and Lutzomyia. The project is divided into four objectives [OJ. 01: To identify and validate potential genes for the development of RNAi in different mosquito borne diseases by shotgun sequencing. 02: Soaking assays to test the candidate shRNA targets against the target insects. 03: Optimization of successful molecules by Saccharomyces cerevisiae expression and further evaluated using feeding assays to verify the viability of in vivo supply. 04: Evaluation of bioinsecticide efficiency by genetic tools analysis of locally interventions.