GHTM

Global Health and Tropical Medicine

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Home / Archives for DNA
GHTM Session 2022CCID03p » Back to the future: Fluorescent Acid-fast-Stains for TB-diagnosis and beyond

GHTM Sessions 2022 CCID03 » Back to the future: Fluorescent Acid-fast-Stains for TB-diagnosis and beyond

  • From: 09/02/2022
  • To: 09/02/2022
  • Location: ZOOM | 11h00

Traditional light-microscopy of stained smears may be replaced by fluorescent stains.
The auramine-O Acid-Fast-Stain (AFS) does not stain the cell-wall (mycolic acids), but is a classical DNA/RNA stain, whereby the cell wall only protects the microorganisms from decolorization by the acid. Confirmation of this new concept opens up the possibility to use this stain in different contexts: a) Auramine-O AFS allows the simple detection of Schistosome eggs; b) Auramine-O AFS allows detection of mycobacteria in histological samples; c) newer nucleic acid-stains may increase (broaden) the application of this stain.
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GHTM Session 2022CCID01p » Plasmonic nanoparticles to detect single nucleotide polymorphisms (SNPs)

GHTM Sessions 2022 CCID01 » Plasmonic nanoparticles to detect single nucleotide polymorphisms (SNPs)

  • From: 12/01/2022
  • To: 12/01/2022
  • Location: ZOOM | 11h00

There is an overall increase in life expectancy associated to high costs in health care for an ageing population in the industrialised world. Consequently, there is a demand for fast, cheap and easy to use diagnostic tests that are essential in identifying patients, determining prognosis, monitoring treatment and assessing the efficacy of prevention. Here, nanotechnology has a prominent role, with special emphasis on noble metal nanoparticles. The presentation focuses on alternative nanotechnology-based test-kit for detection of single nucleotide polymorphism as the one associated with lactose intolerance. Methodology, advantages and limitations will be discussed. 
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GHTM Sessions 2022CCID Diagnostics » 1st Semester Calendar

GHTM Sessions 2022 CCI Diagnostics » 1st Semester Calendar

  • From: 04/01/2022
  • To: 07/05/2022
  • Location: ZOOM | 11h00

GHTM Sessions 2022 CCI Diagnostics » Year Calendar » Sharing GHTM’s Cross Cutting Issues Diagnostics’ activities, knowledge and methodologies in this area.
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GHTM Session 2021VBD01p » Hybridisation and the emergence of Candida pathogens

GHTM Sessions 2021 VBD01 » Hybridisation and the emergence of Candida pathogens

  • From: 20/10/2021
  • To: 20/10/2021
  • Location: ZOOM | 11h00

Human fungal infections are a growing problem having the Candida genus among the main causes. This lecture is an overview of the comparative genomic analysis of Candida pathogens, focusing on hybrid lineages and their evolution, addressing i) how spread are hybrids among Candida species, ii) the processes that drive their genomes’ evolution, and iii) the role that hybridisation might have had in the emergence of C. albicans, the major Candida pathogen.
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GHTM Session 2021THOP01 » Implementation of MinION sequencer (Oxford Nanopore Technologies) at GHTM, by Research Assistant Ana Abrantes.

GHTM Sessions 2021 THOP01 » Implementation of MinION sequencer (Oxford Nanopore Technologies) at GHTM

  • From: 08/06/2021
  • To: 08/06/2021
  • Location: ZOOM | 11h00

MinION is a portable device which applies real-time nanopore sequencing technology to nucleic acid analyses. MinION has several applications, namely whole-genome sequencing, targeting sequencing, Metagenomics, RNA sequencing or Epigenetics.
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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