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Home / Publicações / Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

  • Autores: Afonso A, Cappelini LTD, Carlos IZ, Carrilho E, De Sousa FZR, Leão PAGC, Urbaczek AC, Alberice JV
  • Ano de Publicação: 2017
  • Journal: Scientific Reportsvolume
  • Link: https://www.nature.com/articles/s41598-017-11043-0

This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.

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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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