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Home / Archives for Parreira R

Comparative morphological and molecular analysis confirms the presence of the West Nile virus mosquito vector, Culex univittatus, in the Iberian Peninsula

  • Autores: Almeida AP, Braack L, Bravo-Barriga D, Frontera E, Mixão V, Novo MT, Parreira R, Sousa CA, Venter M
  • Ano de Publicação: 2016
  • Journal: Parasites & Vectors
  • Link: https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1877-7

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Detection of Leishmania DNA and blood meal sources in phlebotomine sand flies (Diptera: Psychodidae) in western of Spain: Update on distribution and risk factors associated

  • Autores: Afonso MO, Blanco-Ciudad J, Bravo-Barriga D, Campino L, Frontera E, Gómez-Gordo L, Maia C, Parreira R, Pérez-Martín JE, Reina D, Serrano FJ
  • Ano de Publicação: 2016
  • Journal: Acta Tropica
  • Link: https://www.sciencedirect.com/science/article/pii/S0001706X16304077?via%3Dihub%20

Leishmaniosis caused by Leishmania infantum is present in Mediterranean countries, with high prevalence in areas of the center and south of Spain. However, in some regions such as Extremadura (in southwest of Spain), data has not been updated since 1997. The aim of this work was (i) to provide information about the distribution of phlebotomine […]
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Zika Virus: A Review to Clinicians

  • Autores: Ferrinho P, Junior V, Luz K, Parreira R
  • Ano de Publicação: 2015
  • Journal: Acta Médica Portuguesa
  • Link: http://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/6929

Zika virus is a flavivirus related to Dengue virus, yellow fever virus and West Nile virus. It is considered an emerging arbovirus transmitted by mosquitos of the genus Aedes. Its first description took place in 1947 in the Zika Forest in Uganda, isolated on Rhesus monkey used as bait to study the yellow fever virus. […]
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Novel multiregion hybridization assay for the identification of the most prevalent genetic forms of the human immunodeficiency virus type 1 circulating in Portugal

  • Autores: Esteves A, Freitas FB, Parreira R, Piedade J
  • Ano de Publicação: 2013
  • Journal: AIDS Research and Human Retroviruses
  • Link: http://online.liebertpub.com/doi/abs/10.1089/aid.2012.0025

The most efficient method for HIV-1 genetic characterization involves full-genome sequencing, but the associated costs, technical features, and low throughput preclude it from being routinely used for the analysis of large numbers of viral strains. Multiregion hybridization assays (MHA) represent an alternative for a consistent genetic analysis of large numbers of viral strains. Classically, MHA rely on the amplification by real-time PCR of several regions scattered along the HIV-1 genome, and on their characterization with clade-specific TaqMan probes (also known as hydrolysis probes).
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Characterization of an insect-specific flavivirus (OCFVPT) co-isolated from Ochlerotatus caspius collected in southern Portugal along with a putative new Negev-like virus

  • Autores: Abecasis A, de Almeida AP, de Matos AP, Esteves A, Lopes A, Parreira R, Piedade J
  • Ano de Publicação: 2013
  • Journal: Virus Genes
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/23877720

We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. The RNA genome of this virus, tentatively designated OCFVPT, for O. caspius flavivirus from Portugal, encodes a polyprotein showing all the features expected for a flavivirus. As frequently observed for ISF, the viral genomes seems to encode a putative Fairly Interesting Flavivirus ORF (FIFO)-like product, the synthesis of which would occur as a result of a -1 translation frameshift event. OCFVPT was isolated in the C6/36 Stegomyia albopicta (= Aedes albopictus) cell line where it replicates rapidly, but failed to replicate in Vero cells in common with other ISFs.
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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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